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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70311411058
中文名称:
英文名称:INCUBATOR GRANT BIO ES-20 SHAKING BENCH TOP 230V 50-60Hz435mm (h) x 340mm (d) x 340mm (w) 25 to
货期:热销品现货,其他请咨询




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文献和实验Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin
Agrobacterium growth and transformation (农杆菌培养及转化)
. 3. Transfer cells + DNA to pre-chilled (on ice) electroporation cuvettes with either1 or 2 mm gap sizes. Make sure the white cuvette holder from the Bio-Rad machine is also pre-chilled on ice. 4. Take the ice bucket with the cuvettes and cuvette
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