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文献和实验Procedures for In Vitro Production of Bovine Embryos
FOR FERTILIZATIONMaterials and Equipment Needed X-Plate HEPES-TALP (pre-warmed) IVF-TALP (pre-warmed and equilibrated in CO2) Sp-TALP (pre-warmed) Percoll gradient (pre-warmed) Dissecting microscope Inverted microscope 60 x 15 petri-dish Rack for tubes (place
Functional Assays on High‐Content Protein Microarrays
/) 50‐ml conical tubes (VWR, cat. no. 89004‐367), sterile Eppendorf centrifuge (5810) (Fisher Scientific, cat. no. 05‐400‐60) with microtiter plate adapter for rotor
In Situ HYBRIDIZATION TO TISSUE SECTIONS
or syringe. Allow the cement to dry. Incubate slides in a humid chamber 18-24h at 37 degrees C (DNA probe) or 50 degrees C (Riboprobe). Post-hybridization washes: DNA probes For every rack of slides, prepare 2L DNA Wash Buffer
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