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- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311738619
中文名称：
英文名称：ROTOR 4X100ML PEAR SHAPED FLASK
货期：热销品现货，其他请咨询
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文献和实验Cool growth competent E . coli
al., 1990). Chill the following: >100 ml ice-cold TB, centrifuge (GSA rotor), 500 ml centrifuge bottle, and sterile freezing tubes (eppendorf tubes w/ good caps) -Innoculate 250 ml SOB media (2 liter flask) w/ 10-12 colonies bacteria
Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Large Scale Tubulin Preparation
. 3. Collect supernatant and transfer 1 liter to a 1.8L glass Fernbach flask that has 500 ml of 37¡C glycerol. Add 0.1 mM GTP, 0.5 mM ATP, and 3.5 mM MgCl2 (this gives 0.1 mM GTP, 1.5 mM ATP and 4 mM MgCl2 final). The ATP and GTP are added as solids
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