赛默飞世尔Thermo Fisher PLT 96WL W/

Procedures for In Vitro Production of Bovine Embryos

hood 100 ml pipet tips and pipettor OMM + supplements Mineral oil 60 x 15 petri dishes Plastic pasteur pipet 3-4 bottles of OCM + supplement (warmed @ 38.5°C) 1 L saline + Pen/Strep (warmed @ 38.5°C)Preparation for Oocyte Collection Scalpel Petri dishes

【求助】蛋白激酶C的信号通路

3BPAkNHeZbAxoknWOPsDS0sDPy3eI6wn0x9tcLz64SWbSH3bJmGW0SOuqItUeSEJZOqnryFHRC9Y3/s83LSUtca28w5545HEtWDLcZh12uP36kSXWs6PLnn76acsWWu2mW+6yb3zrm3bs0ZNt7ty59g9/93UR87Sdfs659vyLL9tQ5bthwxpbsWql1WQi8m+33G4/vOon9o6zTlff6rX7H1gsrx0t9r2rf2JXX3+jffETH7efXX+tXfKej9t1133fvvf9q/x+XPjzm60

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T