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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70310399790
中文名称:
英文名称:X1000 Filter Millex GS filter,0.22µm, non-sterile (pack of 1000)
货期:热销品现货,其他请咨询




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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
Culturing Chick Embryonic Myoblasts/Myotubes and Fibroblasts
3. Afterwards replace medium every third day (no ara c). Ara C Stock (1 mM) 1. Add 1 mg ara c to 2.5 ml distilled H2O. 2. Sterile filter through Millex-GV filter. 3. Store in freezer (-20o C). 4. Use at 5 x 10-6 M in regular skeletal muscle
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