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- 库存：
999
- 供应商：
北京泽平
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热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310238550
中文名称：
英文名称：Orbital shaker KS15A
货期：热销品现货，其他请咨询
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
with the tissue pieces and the collagenase into a tube rack on an orbital shaker inside a 37° C incubator or oven. Turn the orbital shaker on at approximately 60 rpm and close the door. Set a timer for 7 minutes. Be sure to place the collagenase
(Glas-Col rugged rotator, from Fisher). The boxes thus get rotated, pretty much like tubes in a roller drum. Shaking vertically in an orbital would probably be okay too. Equipment required Deepwell titer dishes: Beckman 267006 ($83/24) Capmats
in a GSA rotor (5000 rpm, 5 min., RT). Discard the supernatant and resuspend the pellet in 10 ml T.E. Transfer the cells to a 50 ml centrifuge tube. Pellet the cells in an SS34 rotor (7000 rpm, 5 min., RT). Discard the supernatant and resuspend
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