赛默飞世尔Thermo Fisher POLYESTER L

Cell counting with an hemacytometer.

two 1x1x0.1 mm areas (delimited by a double line; nine small squares), from two chambers. Ideally you should count more than 200 cells and less than 500 per chamber. The number of cells per 1x1x0.1 mm areas = cells x 1*10-4 cm3 (1*10-4 ml). With a 1:20

2-D Polyacrylamide Gel Electrophoresis

into the same microfuge tube. 6.Add 200 µl to the lysates for a total of 400 µl. 7.Vortex vigorously for 1 min. 8.Incubate for 5 min at RT. 9.Vortex vigorously for 1 min. 10.Spin sample down at 14,000 g for 5-10 min at room temp.•To lyse a polyester-embed

Cell counting with an hemacytometer.

for 1-2 min. (If you need to leave for longer, use a humid chamber). This will allow deposition of the cells on the counting plane. Count under the microscope two 1x1x0.1 mm areas (delimited by a double line; nine small squares), from two chambers