赛默飞世尔Thermo Fisher CLAMPING DE

Measurement with the Light Microscope

the focus. Any ocular scale must be calibrated, using a device called a stage micrometer. A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched

A safe method of extracting DNA from Coccidioides immitis

nitrogen, lyophilized, and ground with a mortar and pestle. About 10 mg of dry ground tissue is mixed with 400 ul lysis buffer (50 mM Tris, 100 uM NaCl, 5 mM EDTA, 1 sodium dodecyl sulfate), and incubated at 80 C for 10 min., cooled to 40 C and incubated

用 CD3/CD28 做好 T 细胞激活与扩增

分别利用 CD3 与 CD28 的功能学抗体，以及 CD3/CD28 Streptamer® 激活扩增 T 细胞的实验步骤与实验结果。 CD3/CD28抗体法 1、实验步骤： 1.1 抗体包被 用无菌 PBS 将 anti-mouse CD3 抗体（克隆号：145-2C11）稀释至 5μg/ml，稀释后的抗体加入到 24 孔板中，每孔 400μl，4°C 包被过夜。（注：板子在加入抗体之前，先用无菌 PBS 润洗 2-3 遍，避免干孔）； 1.2 小鼠脾脏淋巴细胞分离（次日） 1. 将 70μm