赛默飞世尔Thermo Fisher Kapillarsäu
le OPTIMA-1 ID: 0,32 mm, Filmdicke: 0, 25 µm, L=50 m 货号：T_70310503792- ￥1200 - 24000
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- 2025年07月15日
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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310503792
中文名称：
英文名称：Kapillarsäule OPTIMA-1 ID: 0,32 mm, Filmdicke: 0, 25 µm, L=50 m
货期：热销品现货，其他请咨询
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Southern Hybridization Protocols
do not have the reproducibility and robustness of the former Boehringer) are used in our laboratory.Place 25ng (1 m l) of template DNA into a microcentrifuge tube. Add in 5 m l of primers and appropriate volume of 50 m l in the final reaction. Denature by heating to 95-100 ℃
Northern Blotting方法(Howell Lab)
inverted because the RNA pellets on the bottom. (DNA can be recovered from the CsCl layer.) Cut off the tube bottoms (keep inverted) and resuspend the pellet in 100 µl 0.3 M NaOAc (pH 6) buffer. Transfer to a screw-cap 1.5 ml Eppi. Wash tube
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