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- 文献和实验
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- 供应商:
北京泽平
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EA
货号:T_70310221300
中文名称:
英文名称:FLUORO FILTER 400 NM EXCITATION EMISSION HBW 10 NM 10 nm
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Fluorochrome Absorption and Emission
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
Fluorochrome Absorption and Emission Spectra
, which are similar in structure to phycoerythrobilin, the chromophore in R-PE. It has a 650-nm wavelength absorption maximum (Fig. 2) and a 660-nm fluorescence emission maximum (Fig. 2). Using a 660 ?10 nm BP filter will give optimum detection for this fluorochrome
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