赛默飞世尔Thermo Fisher QUANTUM IX

Protocol for Trichloroacetic Acid (TCA) Precipitation

and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish

Construction and Characterization of Adenovirus Vectors

. For large-scale preparation of Ad vectors using suspension-adapted 293N3S cells: ix. Distribute a 3-L culture of 293N3S cells into eight 500-mL centrifuge bottles. x. Centrifuge at 650g for 20

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit