赛默飞世尔Thermo Fisher BLUE VERTIC

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

l Gel A 73 µl 86 µl 98 µl 110 µl 122 µl TEMED 8.15 µl 8 µl 7.85 µl 7.71 µl 7.56 µl 10% APS 81.5 µl 80 µl 78.5 µl 77.1 µl 75.6 µl Reagents used in Gels Gel

The ribonuclease protection assay (RPA)

P]UTP 1 µl T7 RNA polymerase (Keep at -20°C until use, return to -20°C immediately). Mix by gentle pipetting or flicking and quick spin in a microfuge. Incubate at 37°C for 1 hour. 2. Terminate the reaction by adding 2 µl of DNase. Mix

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge