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文献和实验Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
, 6.1g anhydrous Na2HPO4, 2g KH2PO4, H2O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.-
V294.PART II,Chapter 2 Boyden法体外趋化试验
is intended specifically for measuring the migration of Madin-Darbycanine kidney cells using a 48-well chamber from Neuro Probe, Inc. Key Words: Boyden chamber assay; migration assay; cell motility; cell migration;chemotaxis; hapatotaxis.
37% formaldehyde: store up to 1 year at room temperature 2.5 M glycine, heat sterilized TBS ( appendix
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