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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
In Situ HYBRIDIZATION TO TISSUE SECTIONS
in xylene, 2 X 10'. Rehydrate through 100%, 95%, 80%, 60% and 30% EtOH; 1' in each solution. Incubate in 0.2N HCl, 20'. Rinse in ddH2O, 30". Incubate @ 70 degrees C in 2X SSC (preheated to 70 degrees C), 30'. Rinse in ddH
Basic procedures for bacteria culture
is dissolved in buffer by heating and the warm gel solution then is poured into a mold (made by wrapping clear tape around and extending above the edges of an 18 cm X 18 cm glass plate), which is fitted with a well-forming comb. The percentage of agarose
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