赛默飞世尔Thermo Fisher 反应壶 REACTIO

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture

Stripping Western Blots

. Add 1ml lysis bufferb and vortex briefly. 11) Repeat Step #10 2-3 more times. 12) After last wash, aspirate supernatant and remove remainder of wash buffer with fine gauge (>22g) needle. Process for kinase reaction (as indicated) or add 40-80μl 1X

Functional Assays on High‐Content Protein Microarrays

. 5:596‐613.    Oláh, J., Vincze, O., Virók, D., Simon, D., Bozsó, Z., Tõkési, N., Horváth, I., Hlavanda, E., Kovács, J., Magyar, A., Szũcs, M., Orosz, F., Penke, B., and Ovádi, J