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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
of plates to be bleached. 6. Rinse the bleached (disinfected) plates with Millipore NANOpure 0.45um-filtered ddH2O (I use the water from our ddH2O storage/wash tank next to the sink). Rinse the bottom plate first under flowing ddH2O (both sides
and very gently squirt a 0.1% SDS overlay over the acrylamide solution (keeps oxygen away so that the top of the resolving gel polymerizes well). If you squirt this on gently enough you won't disturb the acrylamide solution, and a sharp line of refractive index
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
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