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货号：T_70311718609
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英文名称：ROTOR 24X1,5ML FOR DNA SPEED VAC
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文献和实验Purification of DNA from lambda phage
Sodium Acetate pH 4.8 and 2 vol of room temperature 100% ethanol. Mix,microcentrifuge 10 min to precipitate DNA . G.Add 1 ml 70% ethanol and centrifuge 5 min.Dry in speed vac about 5 min,dissolve in 100 pl TE.Yield of DNA should be around 2OItg per 17ml
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
or speed-vac to just dry and resuspend pellets in 1 mM Tris, pH 8.0. Use 10ul of resuspension buffer per ml of starting culture fluid. *Modified, from TIGR protocol Version 052397, Copyright 1997 TIGR. 1 Steps 3 through 8 can be carried
Concentration of DNA by ethanol precipitation
with a more dilute ethanol solution. After a second centrifugation, the supernatant again is discarded, and the DNA pellet is dried in a Speedy-Vac. Protocol 1. Add 2.5-3 volumes of 95% ethanol/0.12 M sodium acetate to the DNA sample contained in a 1.5 ml
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