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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70313435689
中文名称:
英文名称:Column 2.6µm Accucore 150-AMIDE-HILIC 100 x 4.6mm
货期:热销品现货,其他请咨询




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文献和实验.27. Column Buffers Make 500mls equivalent of base buffer: 10ml 1M Hepes pH 7.9 100ml 10% glycerol 5ml 10% NP40 (or 5.0ml 300mM Octyl glucoside) 200ml 0.5 EDTA ------- 115.2ml /5 = 23ml Base 1M KCl2.5M KCl dH20 for 0 KCL 46ml 0 0 154ml = 200
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
at �80°C. (14) Aliquot 100 µl of the supernatants into 1.5 ml microfuge tubes, and add 900 µl of 150 mM NaCl ChIP Dilution buffer/1x proteinase inhibitors. This dilution decreases the SDS concentration in chromatin samples
elution buffer combine supernatants, incubate at 65 °6 h to ON take 1/100 of the protein amount taken for the IP, add to 150 µl elution buffer (INPUT control) incubate at 65 °C 6 h to ON add 750 µl PB buffer (Qiagen
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