赛默飞世尔Thermo Fisher 试管 TUBE W/C

Southern Hybridization Protocols

8.0 20X SSC 50X Denhardts 20% SDS *SS DNA 10 mg/ml16.00 ml 1.25 ml 0.50 ml 6.25 ml 0.50 ml 0.25 ml 0.25 ml 25.00 ml- 50 mM Tris 10mM EDTA 5 x SSC 1 x Denhardts 0.2 SDS 100 m g/ml* Boiled for 10 min then cooled on ice. Then added to the solution at 65

Procedures for In Vitro Production of Bovine Embryos

with OMM + supplement microdrops (pre-equilibrated) Bench paper to cover surface 25 ml pipet automatic pipettor Mesh or styrofoam on counter X-plate Integrid petri-dish100 μm Falcon Cell Strainer50-ml Centrifuge Tubes, self standing with plug seal cap

小RNA的Northern BLOT

-833) Temed (BioRad cat# 161-0800) SafetyCoat: Baker cat# 4017-01 10% APS, Make a 10% w/v in DEPC treated H2O. Ammonium persulfate, Sigma A7460. Keep in refrigerator, make fresh every week. 10xTBE: (890 mM) 216g Trisbase Fisher