赛默飞世尔Thermo Fisher Capillary c
olumn OPTIMA-35 MS ID: 0.32 mm, film thickness: 0.25 µm length: 30 m, 1/PK, MA: 货号：T_70312452840- ￥1200 - 24000
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- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70312452840
中文名称：
英文名称：Capillary column OPTIMA-35 MS ID: 0.32 mm, film thickness: 0.25 µm length: 30 m, 1/PK, MA:
货期：热销品现货，其他请咨询
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文献和实验Paper GB002 (Schleicher %26amp; Schuell, 34550) Gel Blot Paper GB004 (Schleicher %26amp; Schuell, 34340) Whatman paper, 3MM Contrad 70 detergent (Fisher, 04-355-1) 8 x 10 film cassette (Fisher, FB-XC-810) 14 x 17 film cassette (Fisher, FB-XC-1417 8 x 10
buffer, nucleotide extension mix, either [a-32-P]dATP or [a-35-S]dATP, and Bst polymerase) are added. Reactions are performed as described above for single-stranded sequencing.ProtocolFor single-stranded DNA sequencing:1. Prepare the following extension
by heating on a Hoefer vacuum gel drier, and exposed to X-ray film. Depending on the intensity of the signal and whether the radiolabel is 32-P or 35-S, exposure times varied from 4 hours to several days. After exposure, the films are developed by processing
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