赛默飞世尔Thermo Fisher GC COLUMN O

Clonality - X Chromosome Inactivation Assay

(1:1:1, v:v:v) (Gibco) 3 M Na Acetate, pH 5.2 (Life Technologies) Isopropanol Glycogen, 10 mg/ml (GenHunter) 70% ethanol Buffer 1 (50 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 50 mM NaCl) Hha I restriction enzyme (Life Technologies) REact 2 Buffer

Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis

for longer than 3 weeks 0.5 M Tris-HCl, pH 9.5 For 100 ul: 1 mM EDTA 10 mM Spermidine T4 PNK    30 Units

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);