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- 库存:
999
- 供应商:
北京泽平
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货号:T_70313528470
中文名称:
英文名称:SMARTBLOCK 2.0ML FOR 24 TUBES 2.0 ML
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文献和实验Immunohistochemistry: Fluorescence Protocol 2.0
. Sample Preparation and Fixation Harvest cells and wash twice in V-bottomed tubes with cold Wash Buffer by centrifugation (400 x g for 5 minutes) at 4°C to remove extracellular cytokines. Add 0.5 mL of the Fixation Buffer
Replication timing by density transfer
4 , 2.0 M NaCl, pH 6.8 T10 E100 Add to 350 ml dH2 O: 18.61 g Na2 EDTA?H2 O 1.5 g NaOH pellets 5 ml 1M Tris-HCl pH 7.5 Stir until the solids have dissolved, bring the pH to 7.5 with concentrated NaOH (2 O
Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
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