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文献和实验Monoclonal Antibody Production Protocol
. It is advisable to do this spin at the same time that the second wash of the spleen cells is done. The fusion The washed myeloma and spleen cells are pooled in 30ml of PBS (room temp.) and spun gently at 1000 rpm for 10 min. Aspirate the PBS and resuspend
to do this spin at the same time that the second wash of the spleen cells is done. The fusion The washed myeloma and spleen cells are pooled in 30ml of PBS (room temp.) and spun gently at 1000 rpm for 10 min. Aspirate
Basic procedures for bacteria culture
microcentrifuge tube, invert to mix, and incubate in an ice-water bath for at least 10 minutes. It is possible to place the sample at -20degC overnight at this stage. 2. Centrifuge at 12,000 rpm in a microcentrifuge (Fisher) for 15 minutes at 4 degC, decant
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