赛默飞世尔Thermo Fisher OPTIMA-1701

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume:       1.5 ml 1M Hepes       1.05ml 4M NaCl       3ml 10% Triton X (Tx-100)       300µl 10% NAD       24.15ml H20 ChIP high salt buffer       30ml final volume:       1.5ml 1M Hepes    

基于PCR技术的染色质沉淀分析

µCi/µl, specific activity 3000 Ci/mmol) (for hot-stop PCR [Steps 1-22] or SSCP [Steps 23-37] only)dNTPs (stock solutions at 25 mM for each dNTP)DNA loading buffer (6X)30% (v/v) glycerol0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FFStore

Single Primer ("Semi-Random") P

a biased ratio of Po and Pn. Make 3 reactions with varying [Mg++]: 15 µL H2O 3 µL 10x H, M or L-PCRB 3 µL 4 dNTPs @ 2 mM each 3 µL Po @ 5 µM 3 µL template diluted to about 1 ng/µL 3 µL "T/TS" @ 0.4 u Taq/µL -------- 30 µL