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文献和实验and to the vacuum. Filter the lysate through the Whatman,changing it often as it gets clogged. Filtrate may now be stored a 4℃ until it is time to run over an affinity column. Note: Don't store for more than a couple of days at 4℃. References K.D.Puri,E.B
. 8.Insert prbe t the tube and the cver the neck f the tube and the main bdy f the prbe with parafilm.This is very imprtant.This prevents aersls frm escaping during the hmgenizing prcess. 9.Hmgenize in small burst t avid heating the sample. Nte 1: Hmgenize
Large-scale yeast transformation
to use. We use selection because we are maintaining a plasmid. Presumably the main thing is that cells not be stationary. If you are going to plate transformations, a larger pellet could be used - up to 10 ul? Boehringer 146-7-140
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