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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70105-586
中文名称：
英文名称：CENTRIFUGE BOTTLE 250ML 12/CS
货期：热销品现货，其他请咨询
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文献和实验SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
by inverting the tube a few times. Incubate 5 minutes at room temperature. Invert the tube several times during incubation. NOTE: ERL Buffer is supplied as a 10x concentrate and must be diluted with ddH2 O according to bottle label. 2. Centrifuge
into 12 X 75 mm Falcon tubes containing 1.5 ml aliquots of the cells. Incubate for 4-6 hours at 37degC with shaking at 250 rpm. 2. Transfer the culture to 1.5 ml microcentrifuge tubes and centrifuge for 15 minutes at 12,000 rpm at 4degC.
Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
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