赛默飞世尔Thermo Fisher X10 LAB BOT

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

Sauer:RNA Purification from E. coli

too low in RNA prep''d by resuspending E. coli in tri-reagent (called Trizol from here on) as the "lysis" step. Even though Trizol has phenol and guanidinium thiocyanate, and the samples were heated to 65 degrees for 30 minutes, the large RNAs just didn''t

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially