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文献和实验Differential Plant Proteome Analysis by Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)
Protein relative quantitation is one of the main targets in many proteomic experiments. Among the range of techniques available for both top-down and bottom-up approaches, i sobaric t ags for r elative and a bsolute q uantitation (iTRAQ
and sensitive for the quantitation of microgram quantitites of protein utilizing the principle of protein-dye binding.Analytical Biochemistry 72: 248-254.1976.•Stoscheck,CM.Quantitation of Protein.Methods in Enzymology 182: 50-69 (1990).
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
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