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货号：T_70122-146-600
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英文名称：PIPETTE OVATION 0.2UL-2UL
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文献和实验Large-scale yeast transformation
. Resuspend yeast in the 90 mls LiAc/TE, put back in same flask (rinsed with sterile water). Shake at 30o C for 30 mins. Add 600 ul beta-mercaptoethanol. Shake at 30o C for 30 minutes. Add 1.7ml of 10mg/ml ss carrier DNA 3 . Cells ready
Large-scale yeast transformation大规模转化酵母【Yale University】
is that cells not be stationary. 2.If you are going to plate transformations, a larger pellet could be used - up to 10 ul? 3.Boehringer 146-7-140. We omitted this for months. It didn't seem to affect transformation efficiency at all, but maybe it affects non
is that cells not be stationary. If you are going to plate transformations, a larger pellet could be used - up to 10 ul? Boehringer 146-7-140. We omitted this for months. It didn't seem to affect transformation efficiency at all, but maybe it affects non
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