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货号：T_701MACR08124
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英文名称：MULTISCRN CARRIER RCK 4M 100PK
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文献和实验AFFINITY PURIFICATION OF ANTIBODIES
AFFINITY PURIFICATION OF ANTIBODIES (Fisher et al.,1988, Cell 54: 813-822) Conjugation of carrier (BSA) to peptide 1. Dissolve pure BSA @ 20mg/ml in 10mM phosphate, pH 7.0. 2. Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide
. and resuspend in 1.5mL TE/LiAC. Add 100ul cells to the plasmid DNAs (1-5 ug) and single-stranded carrier DNA (20ug) in a microfuge tube. The carrier DNA is produced by dissolving salmon sperm DNA in TE, sonicating it to reduce its viscosity, extracting
. and resuspend in 1.5mL TE/LiAC. Add 100ul cells to the plasmid DNAs (1-5 ug) and single-stranded carrier DNA (20ug) in a microfuge tube. The carrier DNA is produced by dissolving salmon sperm DNA in TE, sonicating it to reduce its viscosity, extracting
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