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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70311786253
中文名称：
英文名称：X10 BOTTLE REAGENT 500ML CN 45/40
货期：热销品现货，其他请咨询
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文献和实验off with 0.05% Tween 20 in PBS. 7. Block for about 1 hr with 5% BSA/Tween 20, or overnight with 3% BSA/Tween 20. Reagents and Buffers for Westerns Reagent 30% acrylamide/bisacrylamide (29:1 mix) 100ml(dark bottle at 4°C) 29g acrlamid 1g bisacrylamid
The production of monoclonal antibody by hybridoma
powder supplied by SIGMA at -700C until needed. Reconstitute 1 vial / 500 ml of media and add entire contents to 500 ml media (eg. 2 vials/ litre). Myeloma Media is CM which has 10% FBS (or HS) and 8-Aza (1 X) stored
containing 0.5 mL heptane and 0.5 mL methanol. For large collections, transfer embryos to a 500 mL bottle containing 100 mL heptane and 100 mL methanol. Devitellinize the embryos by shaking vigorously for 45 s until most of the embryos sink
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