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999
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北京泽平
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货号:T_70110-229
中文名称:烧瓶
英文名称:FLASK VOL PTF STOP 10ML 12/CS
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
]. PLANT GROWTH: 1. Take seeds with a brush and place them into 8cm square pots filled with soil. Don''t compress the soil too much and water the pots thoroughly with 2-3 pot-vol to remove excess nutrients. Place 12-16 seeds in each pot. Place
In Situ HYBRIDIZATION TO TISSUE SECTIONS
ml glass tube -Spot 10ml on a GF/C filter disk -To the remaining 90ml, add 5ml cold 10% TCA; incubate 10' on ice -Filter onto GF/C, wash with 10% TCA, wash with 95% EtOH and dry -20-50% incorporation is good and corresponds to a S.A. ‰ 1-2 x 108
myelin basic protein (UBI or GIBCO)) per assay and incubate on a shaking platform at RT for 30 min. 11) Add 13 μl 4 X SDS sample buffer and boil for 5 min to stop the reaction. 12) Separate reaction by SDS PAGE: 15% to analyze incorporation of 32
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