赛默飞世尔Thermo Fisher 波士顿圆瓶 BTL B

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

(Fisher) Heating mantles (VWR) 100‐, 250‐, and 2000‐mL Erlenmeyer flasks (Kimax

单细胞基因表达分析

for labeling buffer (Cat. No. BP328-1), 25-ml pipets for make-shift size-exclusion chromatography columns (Cat. No. 13-674-41E), and Pasteur pipettes (Cat. No. 13-678-20D) for filling the columns were from Fisher. Sephadex G-50 resin (Cat. No. G-50-150

Single Cell Gene Expression Profiling: Multiplexed Expression Fluorescence in situ Hybridization (FISH)

) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488