赛默飞世尔Thermo Fisher 10-20ML TES

仪器常用词汇

(medicine spoon)×1 (Erlenmeyer flask) 100 ml×3 滴管(drip tube;dropper)×2 烧杯(beaker) 500 ml×1 玻棒(Glass stic)×2 250 ml×3 木试管夹(test tube clamp;test tube holder)×1 100 ml×3 胖肚吸管(straws) 25 ml×1 50 ml×2 10 ml×1 容量瓶(volumetric flask) 100 ml×2 乳钵(morta)×

Purifying Large E. coli Restriction Fragments from Pulsed-Field Gels

razor blade, slice each plug in half, then slice each half-plug in half again, lengthwise. 3-Place two slices (which together would make up one half-plug) inside a sterile Eppendorf tube containing 900 µl sterile 10 mM Tris-HCl (pH 7.5). The pieces

Freezing Worms

with a sterile pipette. 6. Pipette 3 ml FS + agar into a tube of worms and immediately mix by inversion of tube 8-10X. 7. Pipette 1.8 ml of the mixture into 3 prepared freezer vials. 8. Repeat steps 6 and 7 for each strain. 9. Freeze slowly to -80