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货号：T_70114-282-75
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文献和实验can be increased to 75 mM in the Elution buffer if final DNA yields are consistently low). 5. Add 0.6 ml of Elution buffer and collect the flow-through in sterile eppendorf tubes placed in a Sarstedt rack. Transfer a 10 µl aliquot into another eppendorf tube
Procedures for In Vitro Production of Bovine Embryos
OF FERTILIZED OOCYTES INTO EMBRYO CULTURE DROPS (8-10 h post IVF)Materials and Equipment Needed Vortexer Timer 1.5 ml Dolphin microcentrifuge tube and rack X-plate (with prewarmed HEPES-TALP) Plastic Pasteur pipet Heater (placed in front of the microscope) Slide
In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
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