赛默飞世尔Thermo Fisher X100 Mini b

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性

electrophoresis equipment. 2. Transfer buffer: 25 mM Tris, 192 mM glycine, and 10% (v/v)methanol, pH 8.3. 3. Gel blot paper. 4. Polyvinylidene difluoride (PVDF) membrane (Millipore,Billerica, MA). 5. Methanol. 6. Tris-buffered saline (TBS): 10 mM

Methods for DNA sequencing

-HCl pH 8.5 (dilute from 1 M stock), 1.5 mM MgCl2 (dilute from 1 M stock), 0.2 mM of each dNTP (dilute from 100 mM stock), and 5 units of PE-Cetus Amplitaq in 0.5 ml snap cap tubes for 25 cycles of 95oC for 1 min., 55oC for 1 min. and 72oC for 2 min