赛默飞世尔Thermo Fisher DWP96/500 D

Methods for DNA isolation

7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal

细胞/组织中的DNA定量（Quantification of DNA in cell / tissue samples）

・ PE HTS 7000 Bio Assay Reader・ Two filters to provide an excitation wavelength of approximately 360 nm with a band with of 100 nm and to detect the emission at 465 nm・ 96 well white plates・ DNA standardCalf Thymus DNA Sigma ‘ultra pure’,cat.# D-4764

freeshotgun sequencing library from miniprep BAC DNA

, we pick up ~ 400 white colonies to grow and keep in four 96 well microtitres for 1X library. From mentioned 20 plates for each BAC clone, we have 4 to 8 thousands white colonies for generation of 2X, 3X , even 4X shotgun sequencing library without E.coli