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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
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货号：T_701AA30086DR
中文名称：
英文名称：AL23 TUBE (1 END CLSD 50 200MM
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文献和实验BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod
into 50 ml Corning centrifuge tube and DNase-free RNase A is added to a final concentration of 100 ug/ml from a 20 mg/ml stock and RNase T1 is added to a final concentration of 40 ul/100 ml from a 100 U/ul stock followed by incubation in a 37 deg C water
- normally blunt-end ligation of insert to vector does not reform the restriction site (therefore when you design the oligo that make sure it doesn't reform the restriction site when ligated), therefore only religated vector is digested. See one-tube PCR
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
7.6, 50 mM EDTA, pH 8.0), and 1.8 ml of 7.5 M KOAc (note: the 7.5 M KOAc solution is used without pH adjustment) is added into each bottle and transfered to a 50 ml Sorval centrifuge tube. 8、After mixing, the tubes were frozen, until solid, at -70
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