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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701AA31608DW
中文名称:
英文名称:AL24 TUBE (BTH ENDS O 40 300MM
货期:热销品现货,其他请咨询




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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Native Chromatin Preparation and Illumina/Solexa Library Construction
5-21. Keep the cells cold. 5. Aliquot 108 cells from Step 4. The final yield of purified CD4+ T cells is ~20%. 6. Centrifuge the cell suspension at 300g for 10 min. 7. Resuspend the cell
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
is supposed to work well in reverse transcriptase buffer, and vice-versa, meaning 1-tube protocols (with cDNA synthesis and subsequent PCR) are possible (Krawetz et al., 19xx; Fuqua et al., 1990)。 Higher than 50mM KCl or NaCl inhibits Taq
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