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北京泽平
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货号:T_701F144058MG
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英文名称:PIPETMAN G P200G STNLESSPIPET
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文献和实验Xia, B., Kawar, Z.S., Ju, T., Alvarez, R.A., Sachdev, G.P., and Cummings, R.D. 2005. Versatile fluorescent derivatization of glycans for glycomic analysis. Nat. Methods 2:845‐850
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.
chamber, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
for the tissue to adhere to the surface; then add 1ml fresh media. The p-dish will be your back-up if the coverslips don't grow or get contaminated. D . Allow the tissue in the centrifuge tube to settle while you prepare and sterile filter the collagenase
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