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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_7010340532
中文名称：瓶250ML的12/PK
英文名称：BOTTLES GRADUATED 250ML 12/PK
货期：热销品现货，其他请咨询
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文献和实验Transformation of E. coli by Electroporation
autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Purification of acidic phosphatase from mustard seedlings
6. Centrifuge in the Sorvall superspeed centrifuge (B 7202) in GSA rotor (r = 12.5 cm) at 9500 rpm (1,000 x g) for 30 min. 7. Decant the supernatant (SN1) (contains many organelles and the cytosol) into a graduated cylinder and note the volume. Remove
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