赛默飞世尔Thermo Fisher E10ST STERI

In-Vitro Adipocytes Differentiation

lines (clonal lines 3T3-L1, 3T3-F442A, Ob17, BFC-1, ST13, A31T, ...) and primary culture of adipocyte precursors and pre-adipocytes. Growth arrest of adipoblasts at the G1/S phase of the cell cycle appears to be required for the commitment of adipoblasts

Preparation of Embryonic Extract

bottle. 4. 2 sets of 100 mm sterile petri dishes (glass or plastic) on ice. 5. 1x 50 ml sterile beaker, keep on ice. 6. 3x 250 ml sterile beakers, keep on ice. 7. Cheese cloth, sterilized by autoclaving. 8. 250 ml Saline G, on ice

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

is the same as that in STA-PCR. Fig. 1. Schematic of strategies for cloning genomic DNA sequence flanking a proviral insertion. (a) STA-PCR protocol. Genomic DNA (horizontal lines) is digested with a cocktail of restriction enzymes (noted as E, F, and G