赛默飞世尔Thermo Fisher Column clam

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

寡核苷酸纯化

with the syringe plunger. Carefully remove the plunger and rinse with 3 ml of TE. Remove the syringe and incubate the crushed gel slice/TE on the tube roller at 37 degrees for ~ 4 hr or overnight. 4. Dump the crushed gel slice solution to a Fisher screening column

Oligonucleotide Purification

to a Fisher screening column which has been placed in a 15 ml conical tube. Centrifuge for 1-2 min in clinical centrifuge. This step removes large pieces of acrylamide. 5、 Add the equivalent of 0.3 ml packed DEAE sepharose FF resin to an empty BioRad spin