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北京泽平
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- 保修期：
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货号：T_70311888541
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英文名称：30M SAFETY WRAP DIAMETER 3/4""
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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
RSP-1T 1 1" diameter post, 1" height Newport PS-1 1 1" diameter post
, open film cassette, put 1 piece of X-ray film (Kodak X- Omat R film, XR-5) on intensifying screen, insert filters oriented towards the film, seal cassette and wrap in foil (safety lights can be on). Expose film at -70℃. Remove film (safety lights
Screening lambda cDNA or genomic libraries
and store at 4°.It's best to keep things clean during the lifts so that fungus doesn't end up growing on your plates. Alkaline denaturation - pull off filter,lay DNA side up in 1.5 M NaCl,0.5 M NaOH 30-60 sec - transfer to 1.5 M NaCl,0.5 M Tris pH
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