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货号：T_70310707841
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英文名称：REDUCING PIECE DN 25/10
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of mannitol over the electrode chamber can not be used for longer that 10 - 15 minutes (it evaporates and the fusion becomes less efficient) and should be changed to fresh one after that time. Pass the group of 20-25 embryos through the drop of mannitol
PRODUCTION OF ES CELL CHIMERAS BY AGGREGATION WITH EIGHT-CELL STAGE EMBRYOS
Grip 35 x 10 mm, Falcon 3001-3); KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410); aggregation (darning) needle ( DN-09, BLS Ltd., Hungary ,) 70% ethanol. Method: 1. Place few rows
, without mercuric chloride? [ B-5 is: Water 90 ml Formalin (40% HCHO) 10 ml Mercuric chloride 6 g Sodium acetate (anhydrous) 1.25 g The sodium acetate brings the pH into the 5.8-6.0 range. Fix by immersion, 12-24 hours, then transfer to 70-80% alcohol
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