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文献和实验V294.PART II，Chapter 2 Boyden法体外趋化试验
of ethylene diamine tetraacetic acid (0.53 mM) and 0.01 g of phenolred in 1 L of phosphate-buffered saline, pH 7.4. 7. 2.5% (w/v) Trypsin (cat. no.15090-046; Gibco Invitrogen): dilute 1:25 in Versenebefore use. 8. Collagen from calf skin (cat. no. C
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
) supplemented with 10% (v/v) foetal bovine serum (FBS; Gibco; Invitrogen, Carlsbad, CA), penicillin/streptomycin(Gibco) and 10 μM retinoic acid to induce neuronaldifferentiation (see Note 1 ). 2. Insulin-like growth factor (IGF)-1 (Invitrogen) was dissolvedin
Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
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