赛默飞世尔Thermo Fisher SUCCION SYR

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

寡核苷酸纯化

with the syringe plunger. Carefully remove the plunger and rinse with 3 ml of TE. Remove the syringe and incubate the crushed gel slice/TE on the tube roller at 37 degrees for ~ 4 hr or overnight. 4. Dump the crushed gel slice solution to a Fisher screening column

Oligonucleotide Purification

column. Add 1.5 ml TE. Place column in 13 x 100 mm glass test tube. Spin in clinical centrifuge (12-place fixed angle rotor) on setting 2 for ~1 min until all liquid is out of the resin (don't over centrifuge as this may damage the resin). 6、 Add 1.5 ml