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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_701K179700-0819
中文名称:
英文名称:ADAPTER UNIVERSAL 5-8MM 19/22
货期:热销品现货,其他请咨询




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文献和实验AFLP: not only for fingerprinting, but for positional cloning
-adapter 1.0 µL MseI-adapter 1.0 µL 10 mM ATP 4.0 µL 5x-OPA+-BSA 1 U T4 ligase (Pharmacia) Q.S. to 10 µL w/ dH20 - Incubate @ 37° C for 3 hrs to O/N 2.0 Pre-amplification of template DNA [Note 1: This pre-amplification step helps to "clean
AFLP: not only for fingerprinting, but for positional cloning
. Extraction Buffer 50 mM Tris, pH 8.0 10 mM EDTA, pH 8.0 100 mM NaCl 1.0 % (w/v) SDS 10 mM ß-mercaptoethanol (add just before use) 3) Add 150 µL 5 M K-acetate, vortex, incubate on ice for 20 min. Spin @ 12K x g
Digital Gene Expression by Tag Sequencing on the Illumina Genome Analyzer
) 5 U/µl T4 DNA ligase (Invitrogen) DNA Away (Molecular BioProducts) 32 mM (800×) S‐adenosylmethionine
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