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文献和实验Evaluating Modulators of “Regulator of G‐protein Signaling” (RGS) Proteins
. Described in this unit are high?throughput screening procedures for identifying modulators of RGS protein?mediated GTPase acceleration (GAP activity), for assessment of RGS domain/G? interactions (most avid in vitro when G? is bound by aluminum tetrafluoride
A quick RNA mini-prep for Neurospora mycelial cultures
volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
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