赛默飞世尔Thermo Fisher 标签胶带纸 TAPE

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

of 70‐ to 250‐ml volume (e.g., CBS Scientific GM‐150 or GM‐200 or Hoefer SG500) Magnetic stirrers and stir bars Graduated cylinder Adhesive tape 1.5‐mm Teflon combs (10‐, 15‐, or 20‐tooth

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2

Differential Display of Cotton Transcripts

, Madison, WI) 20 uM decamer primers (random) 20 uM anchored oligo-dT12MN primers (M=A,C, or G; N=A,C,G, or T) mixed dNTPs (2.5 mM each dNTP, 100 uM each dNTP Taq DNA Polymerase and buffer (Promega, Madison, WI